Proteomics and phosphoproteomics analysis of Haematococcus pluvialis

Hazel Hall on the University of Maryland Eastern Shore campus

Program, Project, or Expedition Name:

Proteomics and phosphoproteomics analysis of Haematococcus pluvialis: An improved method to generate and interpret proteomics and phosphoproteomics data of a non-model species

Data Link: https://www.ebi.ac.uk/pride/archive/projects/PXD054975


InstitutionLMRCSC, UMCES
Principal InvestigatorYantao Li | yantao@umces.edu
Funding Agency/Contract #NOAA Office of Education Educational Partnership Program (NOAA EPP) award NA16SEC4810007
Collection Start Date03/01/2024
Objectives of Data Collection EffortHarvest green stage and red stage Haematococcus pluvialis cultures to have two data sets to test using the improved data analysis method being developed for this paper.
Collection End Date04/01/2024
Data Collectors Identifying NumbersN/A
Ship or Other Platform NameN/A
Geographic Location [Latitude(s)/longitude(s)]N/A
Unitsp-values
Data ParametersProtein expression fold-change
PrecisionFDR 0.01
Observation MethodologyLC-MS/MS
Instrument/Gear  Identification of DescriptionDionex 3000 RSLC nano-scale liquid chromatograph in tandem with an Orbitrap Exploris 480 mass spectrometer
Analysis MethodologyProtein expression analysis and proteome annotation
Data Processing/ Reduction MethodologyExcel, Unix, DAVID, Blast2GO, KEGG
Explanations of Data Quality FlagsN/A
Citations of Relevant Publications and Grey LiteratureRamarui, K., Li, Y. Proteomics and phosphoproteomics analysis of Haematococcus pluvialis: An improved method to generate and interpret proteomics and phosphoproteomics data of a non-model species. J Appl Phycol 36, 3243–3260 (2024). https://doi.org/10.1007/s10811-024-03356-1
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